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A coronavirus detected in the vampire bat Desmodus rotundus BJID
Brandão,Paulo Eduardo; Scheffer,Karin; Villarreal,Laura Yaneth; Achkar,Samira; Oliveira,Rafael de Novaes; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Kotait,Ivanete; Richtzenhain,Leonardo José.
This article reports on the identification of a group 2 coronavirus (BatCoV DR/2007) in a Desmodus rotundus vampire bat in Brazil. Phylogenetic analysis of ORF1b revealed that BatCoV DR/2007 originates from a unique lineage in the archetypical group 2 coronaviruses, as described for bat species elsewhere with putative importance in Public Health.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coronavirus; Vampire bat; RNA-dependent; RNA-polymerase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600003
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Desmodus rotundus and Artibeus spp. bats might present distinct rabies virus lineages BJID
Fahl,Willian Oliveira; Carnieli Jr,Pedro; Castilho,Juliana Galera; Carrieri,Maria Luiza; Kotait,Ivanete; Iamamoto,Keila; Oliveira,Rafael Novaes; Brandão,Paulo Eduardo.
In Brazil, bats have been assigned an increasing importance in public health as they are important rabies reservoirs. Phylogenetic studies have shown that rabies virus (RABV) strains from frugivorous bats Artibeus spp. are closely associated to those from the vampire bat Desmodus rotundus, but little is known about the molecular diversity of RABV in Artibeus spp. The N and G genes of RABV isolated from Artibeus spp. and cattle infected by D. rotundus were sequenced, and phylogenetic trees were constructed. The N gene nucleotides tree showed three clusters: one for D. rotundus and two for Artibeus spp. Regarding putative N amino acid-trees, two clusters were formed, one for D. rotundus and another for Artibeus spp. RABV G gene phylogeny supported the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rabies Bat Artibeus spp.; Desmodus rotundus; Phylogeny; Molecular epidemiology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600008
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Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples BJID
Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete.
Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008
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Short-interfering RNAs as antivirals against rabies BJID
Brandão,Paulo Eduardo; Castilho,Juliana Galera; Fahl,Willian; Carnieli Jr.,Pedro; Oliveira,Rafael de Novaes; Macedo,Carla Isabel; Carrieri,Maria Luiza; Kotait,Ivanete.
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
Tipo: Info:eu-repo/semantics/article Palavras-chave: SiRNA; Rabies; Antiviral.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200011
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Species determination of Brazilian mammals implicated in the epidemiology of rabies based on the control region of mitochondrial DNA BJID
Carnieli Junior,Pedro; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Brandão,Paulo Eduardo; Carrieri,Maria Luiza; Kotait,Ivanete.
Identification of animals that are decomposing or have been run over or burnt and cannot be visually identified is a problem in the surveillance and control of infectious diseases. Many of these animals are wild and represent a valuable source of information for epidemiologic research as they may be carriers of an infectious agent. This article discusses the results obtained using a method for identifying mammals genetically by sequencing their mitochondrial DNA control region. Fourteen species were analyzed and identified. These included the main reservoirs and transmitters of rabies virus, namely, canids, chiroptera and primates. The results prove that this method of genetic identification is both efficient and simple and that it can be used in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondrial DNA; Sequencing; Identification; Mammals; Rabies.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600002
Registros recuperados: 5
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